Fig 1: Activation of transcription factor NF-kB in the cells in the culture upon treatment with V. vinifera pruning waste subcritical water extract (SWE) and resveratrol in the concentration of 25 µg/mL (light gray), or 50 µg/mL (black) for 48 h. Results expressed as percent of control NF-kB p65 presence in nuclear extracts of HeLa, MRC-5 and MCF-7 cell lines, determined by ELISA p65 assay (Abcam, Cat. No. ab133112). Nuclear extracts obtained by Nuclear Extraction Kit (Abcam, Cat.No. ab113474). Protein concentration determined prior to ELISA NF-kB p65 assay using a BCA Protein Quantification Kit (Abcam, Cat. No. ab102536).